Molecular Typing of Methicillin Resistant Staphylococcus Aureus Strains Isolated from Mastiticdairy Cows

The aims of this study were to isolate and identify of methicillin resistant Staphylococcus aureus (MRSA) from mastitic cow milk. The investigation presence of mecA genes and molecular characteristics of these isolates using eight primers of repetitive element palindromic-PCR (Rep-PCR) analysis were achieved. Ninety six milk samples, collected from clinical mastitic cows from Sharkia Governorate in Egypt, were obtained from 71cowcases. MRSA isolates were recovered by pulsed field gel electrophoresis (PFGE), staphylococcal chromosomal cassette (SCC) mec typing, staphylococcal protein A. A total of 11 MRSA isolates were identified. All strains were showed a positive mecAI genes band (with size about 162 bp long) and mecAII gene band (with size about 449 bp long) and negative in mecAIII gene band (with size about of 540 bp long). The results of Rep-PCR analysis for the 11 MRSA isolates revealed approximately 114 different banding pattern, 51 of them consider as monomorphic bands with a percentage about 44.8%, and other 63 fragments consider as polymorphic bands with about 55.2%. The dendogram based on banding patterns dividing the 11 isolates into two wjpls, 2016, Vol. 2, Issue 6, 567-582 Research Article ISSN 2454-2229 World Journal of Pharmaceutical and Life Sciences WJPLS www.wjpls.org SJIF Impact Factor: 3.347 *Corresponding Author Dr. Mohamed M. Hassan Scientific Research Deanship, Taif University, Saudi Arabia.